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Tuesday, April 28, 2020 | History

2 edition of Biodegradation of chlorinated methanes using a methylotrophic/anaerobic biofilm reactor found in the catalog.

Biodegradation of chlorinated methanes using a methylotrophic/anaerobic biofilm reactor

Stephen J. Celeste

Biodegradation of chlorinated methanes using a methylotrophic/anaerobic biofilm reactor

  • 393 Want to read
  • 2 Currently reading

Published .
Written in English

    Subjects:
  • Bioreactors.,
  • Biofilms.,
  • Methylotrophic microorganisms -- Biotechnology.,
  • Organochlorine compounds -- Biodegradation.

  • Edition Notes

    Statementby Stephen J. Celeste.
    The Physical Object
    Pagination38 leaves, bound :
    Number of Pages38
    ID Numbers
    Open LibraryOL15172790M

    Organics and suspended solids. The BOD, COD and SS of various anaerobic treatment systems anaerobic ponds, UASB reactors, Imhoff tank and septic tanks treating sewage without any post treatment system has been reported to vary from 60 to ; to and 50 to mg/L, respectively (Chernicharo, ; Foresti et al., ).The process efficiency depends on Cited by: Toxicity in Anaerobic Biodegradation of Vegetable Oil in Freshwater Sediment COOGER, Fisheries and Oceans Canada methane Outflow Tygon tube (3 ft) for evolved gases Canola Oil and Methane Quantification flushed with N 2 Culture medium sediment CO 2 trap with M NaOH Closed Reactor for Anaerobic Degradation of. z Microtox® Solid-Phase. Accurate O 2 delivery enabled benzene biodegradation through aerobic activation followed by Bioreduction of Antimonate by Anaerobic Methane Oxidation in a Membrane Biofilm Batch Reactor Bromate and Nitrate Bioreduction Coupled with Poly-β-hydroxybutyrate Production in a Methane-Based Membrane Biofilm Reactor Lai, C. Y., Lv, P. L.


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Biodegradation of chlorinated methanes using a methylotrophic/anaerobic biofilm reactor by Stephen J. Celeste Download PDF EPUB FB2

This study explored the use of a combination of methylotrophic and anaerobic bacteria to biodegrade chlorinated methanes. The objectives were to develop a reactor system capable of sustaining both methylotrophic and anaerobic bacterial populations, and to evaluate the biodegradation of chlorinated methanes in this : Stephen J.

Celeste. A sixth chlorinated Biodegradation of chlorinated methanes using a methylotrophic/anaerobic biofilm reactor book, tetrachloroethylene, was not degraded by the methane-utilizing culture under these conditions.

The biodegradation of TCE was inhibited by acetylene, a specific inhibitor of methane oxidation by methanotrophs. This observation supported the hypothesis that a methanotroph is responsible for the observed by: Biodegradation of chlorinated methanes using a methylotrophic/anaerobic biofilm reactor.

Abstract. Graduation date: Chlorinated low molecular weight solvents have been discovered in a\ud number of the nation's groundwater supplies.

Remediation of these aquifers will\ud require some form of treatment. Presently, the dominant treatment. The biodegradability of chlorinated methanes, chlorinated ethanes, chlorinated ethenes, chlorofluorocarbons (CFCs), chlorinated acetic acids, chlorinated propanoids and chlorinated butadienes was evaluated based on literature data.

Evidence for the biodegradation of compounds in all of the compound categories evaluated has been reported. A broad range of chlorinated Cited by: (). Biodegradation of chlorinated methanes using a methylotrophic /anaerobic biofilm reactor. MS Thesis, (). Biodegradation of dichloromethane and its utilization as a growth substrate under methanogenic conditions.

The kinetics were determined by using a completely mixed laboratory biofilm reactor seeded with methane-oxidizing (methanotrophic) bacteria originally obtained from an aquifer contaminated with chlorinated hydrocarbons.

The contaminant degradation followed first order kinetics in the concentration range 0–1 mg/ by: 1. Introduction. As reflected by the vast knowledge about aerobic degradation mechanisms in comparison to anaerobic ones (e.g.

Neilson and Allard, ), aerobic conditions are generally reported to be more effective for the removal of most organic l pollutants, such as highly halogenated aromatic compounds, however, have been shown to be more Cited by: Biodegradation of Chlorinated Compounds—A Review Article (PDF Available) in Critical Reviews in Environmental Science and Technology 37(2).

Biodegradation of chlorinated methanes using a methylotrophic/anaerobic biofilm reactor book of Chlorinated Biodegradation of Chlorinated Solvents Jim A. Field chlorinated solvents by anaerobic sludge 13 Five Physiological Roles 1st: aerobic carbon and energy source methane, toluene, phenol, ammonium, ethane, ethene, propane etc.

Chlorine has been removed from the chlorinated aliphatic compounds by anaerobic conditions. The aim of this research was investigation of biological treatment of VOC's in high flow speed reactors. During anaerobic biodegradation of chlorinated compounds, sequential removal of chloride ions is generally observed.

Figure 1 demonstrates the dechlorination of PCE to TCE to cis-DCE or trans-DCE to VC to the final Biodegradation of chlorinated methanes using a methylotrophic/anaerobic biofilm reactor book product, ethene. In this reaction, hydrogen, the electron donor, is oxidized while the chlorinated ethene, the electron acceptor, is reduced.

This study was conducted to explore the potential for 1,2-Dibromoethane (EDB) biodegradation by an acclimated microbial consortium under simulated dynamic groundwater conditions. The enriched EDB-degrading consortium consisted of Biodegradation of chlorinated methanes using a methylotrophic/anaerobic biofilm reactor book bacteria Desulfovibrio, facultative anaerobe Chromobacterium, and other potential EDB degraders.

The results showed that the biodegradation Cited by: 1. This study explored the use of a combination of methylotrophic and anaerobic bacteria to biodegrade chlorinated methanes.

The objectives were to develop a reactor system capable of sustaining both methylotrophic and anaerobic bacterial populations, and to evaluate the biodegradation of chlorinated methanes in this system. A gas permeable membrane (Goretex) separated the reactor. Employing a special anaerobic membrane biofilm batch reactor (MBBR), we demonstrated antimonate (Sb(V)) reduction using methane (CH4) as the sole electron donor.

Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), and Raman and Cited by: EVALUATION OF ANAEROBIC BIODEGRADATION OF HIGH CONCENTRATIONS OF HALOGENATED METHANES A Thesis Presented to the Graduate School of Clemson University In Partial Fulfillment of the Requirements for the Degree Master of Science Environmental Engineering by Priya Rachel Jacob August Accepted by: Dr.

David L. Freedman. A few studies have demonstrated the applicability of sequential anaerobic-aerobic biodegradation using a microbial consortium and an indigenous microbial community. Among the studies, biodegradation of a single or several similar chemicals, such as chlorinated ethylenes (Miller et al.

; Tiehm and Schmidt ), was by: Anaerobic Biodegradation of Chlorinated Solvents at Pinellas Science, Technology, and Research (STAR) (Largo, FL) An in situ anaerobic bioremediation demonstration was completed in July of for a portion of the groundwater plume at the former DOE's Pinellas Plant in Largo, Florida, now known as Pinellas STAR Center.

Domestic sewage treatment in a pilot-scale anaerobic sequencing batch biofilm reactor was observed by Sarti et al. Data obtained from days of treatment was analyzed, and it was seen that, under stable operating conditions after the startup period, the mean values of COD removal efficiency achieved was 66%.Cited by: Aerobic and Anaerobic Biodegradation This document provides an inrdepth explanation, detailing the processes of aerobic and anaerobic biodegradation.

It is intended for general audiences and will provide the reader with the necessary information to understand what is happening during the biodegradation process. The sludge obtained from the anaerobic digester of a commercial treatment plant was used to obtain an anaerobic consortium capable of partially dechlorinating 2,4,6-trichlorophenol (2,4,6-TCP).

The clarified and sterilized effluent from the same anaerobic digester was used as the medium for the anaerobic consortium. /bitabs The feasibility of using methanotrophs in an attached‐film, fluidized‐bed (MAFFB) reactor system has been under investigation since Mixed culture, methane‐utilizing attached biofilms were developed on diatomaceous earth particles and on granular activated carbon.

The required feed gases, methane and oxygen, were supplied to the attached biofilm. We developed a microbial community capable of biodegrading mixtures of chlorinated ethenes and 1,4-dioxane under varying redox conditions.

Achieving the removal of chlorinated ethenes as well as 1,4-dioxane to below health advisory levels in groundwater that has anaerobic and aerobic zones has proven to be challenging.

However, our mixed community, composed of the anaerobic chlorinated Cited by: 2. anaerobic biodegradation of compounds in groundwater [Toxic Substances Control Act (TSCA) final test rule of J ; 53 Fed. Reg.40 CFR section“Anaerobic microbiological transformation rate data for chemicals in the subsurface environment”].

Determination of Anaerobic and Anoxic Biodegradation Capacity of Sulfamethoxasole and the Effects on Mixed Microbial Culture.

By Zeynep Cetecioglu, Bahar Ince, Samet Azman, Nazli Gokcek, Nese Coskun and Orhan Ince. Submitted: June 27th Reviewed: February 4th Published: June 14th DOI: /Cited by: 6. Biodegradation of Chlorinated Aliphatics and Aromatic Compounds in Total-Recycle Expanded-Bed Biofilm Reactors V.

KORDE, 1 T. PHELPS, 2'3 P. BIENKOWSKI, *'1'2 AND D. WHITE 1'3 1 Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 3 ; 2Department of Chemical Engineering. Abstract.

This research investigated biodegradation of several halogenated methanes under methanogenic conditions. Biodegradation of dichloromethane to C02 and acetic acid (both environmentally acceptable products) was demonstrated in a fixed-film reactor, operated at 20 deg C, a residence time as low as day, and an influent concentration of 91 micro M.

Yen-Hui Lin, Biodegradation of 2,4-Dichlorophenol by Mixed Culture in an Aerobic Fixed-Biofilm Process—Kinetic Model and Reactor Performance, Environmental Engineering Science, 32, 6, Cited by: This chapter outlines fundamental— and often special —characteristics of biofilm systems in the water industry.

Building on these fundamentals, the describes how the water industry uses biofilms as the heart of technology that improves water quality. Finally, it highlights the ways in which biofilms cause problems in the water industry and strategies to counter the by:   mechanism of aerobic & an-aerobic biodegradation presented by: sukhjeet kaur bt(h)3sem 10/5/ 1 2.

CONTENT Biodegradation Types Aerobic degradation Mechanism of aerobic degradation Anaerobic degradation Mechanism of anaerobic degradation Difference between aerobic and anaerobic 10/5/ 2.

The biodegradability of chlorinated methanes, chlorinated ethanes, chlorinated ethenes, chlorofluorocarbons (CFCs), chlorinated acetic acids, chlorinated propanoids and chlorinated butadienes was evaluated based on literature data. Evidence for the biodegradation of compounds in all of the compound categories evaluated has been reported.

A broad range of chlorinated. Enhanced anaerobic bioremediation is the practice of adding hydrogen to soil and groundwater to increase the number and vitality of indigenous microorganisms performing anaerobic bioremediation.

In this process, anaerobic microbes substitute hydrogen for chlorine on chlorinated contaminant molecules, dechlorinating the compound. Anaerobic digestion has been widely applied to treat the waste activated sludge from biological wastewater treatment and produce methane for biofuel, which has been one of the most efficient solutions to both energy crisis and environmental pollution challenges.

Anaerobic digestion sludge contains highly complex microbial communities, which play crucial roles in Cited by: In Situ Remediation Using Anaerobic Biotransformation of Groundwater Contaminated with Chlorinated Solvents. Soluble Methane Monooxygenase Activity in Methylomonas Methanica Isolated from a Trichloroethylene-Contaminated Aquifer.

Trichloroethylene Cometabolism by Phenol-Degrading Bacteria in Sequencing Biofilm Reactors. Enhanced aerobic biodegradation is the practice of adding oxygen to saturated soil and groundwater to increase the number and vitality of indigenous microorganisms able to perform biodegradation.

Oxygen is considered by many to be the primary growth-limiting factor for hydrocarbon degrading bacteria. Use of an appropriate hydrogen level is necessary to favor dehalogenation of chlorinated solvents, such as tetrachloroethene (PCE) and trichloroethene (TCE), over other hydrogen using processes.

This study examined the competition between dehalogenators and other microorganisms occurring in a benzoate-acclimated dehalogenating methanogenic mixed culture.

other treatments. Methanotrophs produce methane monooxygenases (MMOs) which catalyze methane oxidation and cometabolize chlorinated and aromatic compounds. High rate of TCE degradation is attributed to only soluble MMO (sMMO) expressed mainly by type II methanotrophs under copper-deficient conditions.

To make. Biofilms in aquatic ecosystems colonize various surfaces (sand, rocks, leaves) and play a key role in the environment. Aquatic biofilms supply energy and organic matter to the food chain, they are important in recycling organic matter and contribute to water quality.

FINAL REPORT MODELING FOR ANAEROBIC FIXED-BED BIOFII.M REACTORS by Bill Y.M. Liu and John T. Pfeffer DEPARTMENT OF CIVIL ENG1NEERING UNIVERSITY OF ILLINOIS URBANA, ILLINOIS Technical Monitor: Barbara Goodman. A gas permeable membrane divides a reactor vessel into a liquid compartment and a gas compartment.

A biofilm is grown on the gas permeable membrane on the liquid side of the membrane. Teh gas permeable membrane is supported by the structure of the membrane itself. The biofilm is chosen from bacteria to degrade cetain pollutants by means of anaerobic Cited by:   undertaken to validate the use of enhanced anaerobic bioremediation to biodegrade the primarily dissolved-phase solvent plume.

3-D Microemulsion, an injectable, 3-stage electron donor release material was selected as the preferred bioamendment to accelerate the anaerobic biodegradation process. Project Details. Pdf Report: Mechanisms, Chemistry, and Kinetics of Anaerobic Degradation pdf cDCE and Vinyl Chloride EPA Grant Number: RC Subproject: this is subproject numberestablished and managed by the Center Director under grant R (EPA does not fund or establish subprojects; EPA awards and manages the overall grant for this center).

Download pdf attempted to mimic in small upflow anaerobic sludge bed (UASB) bioreactors the metabolic association found in nature between methanogens and methanotrophs.

UASB bioreactors were inoculated with pure cultures of methanotrophs, and the bioreactors were operated by using continuous low-level oxygenation in order to favor growth and/or survival of methanotrophs. Unlike the reactors Cited by: An aerobic, sequencing biofilm reactor using phenol as the growth ebook cometabolism-inducing substrate was developed to treat water contaminated with trichloroethene (TCE) to a level below the US Environmental Protection Agency drinking water standard of 5 {micro}g/L.

Cometabolism of chloroethene mixtures was of interest in this research because.